1WA4
Crystal structure of the M131F L135A EvaD double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-14 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.632, 79.101, 48.777 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.176 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.997 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.160 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.070 | 0.180 |
| Number of reflections | 9756 | |
| <I/σ(I)> | 7.2 | 5.1 |
| Completeness [%] | 98.6 | 95.3 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS, PH8, 150MM NACL, 25%W/V PEG8000, pH 8.00 |
