1W8P
Structural properties of the B25Tyr-NMe-B26Phe insulin mutant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.903, 62.116, 47.796 |
Unit cell angles | 90.00, 110.58, 90.00 |
Refinement procedure
Resolution | 25.570 - 2.080 |
R-factor | 0.192 |
Rwork | 0.189 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1evr |
RMSD bond length | 0.018 |
RMSD bond angle | 1.708 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.560 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.030 | 0.120 |
Number of reflections | 17496 | |
<I/σ(I)> | 6 | |
Completeness [%] | 88.0 | 89 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1 M TRI-SODIUM CITRATE, 0.02 % W/V ZINC ACETATE, 6 % W/V TRIS/HCL PH 8.2, 0.1 % W/V PHENOL. |