1W8H
structure of pseudomonas aeruginosa lectin II (PA-IIL)complexed with lewisA trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.575, 75.824, 52.746 |
| Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
| Resolution | 26.350 - 1.750 |
| R-factor | 0.126 |
| Rwork | 0.125 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.820 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | 0.330 |
| Number of reflections | 34811 | |
| <I/σ(I)> | 13.19 | 3.76 |
| Completeness [%] | 85.1 | 41.7 |
| Redundancy | 4.06 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
