1W8F
PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL)COMPLEXED WITH LACTO-N-NEO- FUCOPENTAOSE V(LNPFV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.015, 48.352, 52.472 |
| Unit cell angles | 85.35, 80.05, 65.60 |
Refinement procedure
| Resolution | 14.510 - 1.050 |
| R-factor | 0.106 |
| Rwork | 0.105 |
| R-free | 0.12300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzt |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.059 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.510 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 180957 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 93.3 | 89.5 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMNONIUM SULFATE, pH 8.50 |
