1W6T
Crystal Structure Of Octameric Enolase From Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 143.702, 143.702, 100.579 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.100 |
| R-factor | 0.15 |
| Rwork | 0.148 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e9i |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.752 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 68907 | |
| <I/σ(I)> | 14.5 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% (W/V) PEG 1000, 0.3 M MGCL2, AND 0.1 M MES PH 7.5 |
