1W57
Structure of the Bifunctional IspDF from Campylobacter jejuni containing Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2002-06-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 107.760, 107.760, 161.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.350 - 3.090 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w55 |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.128 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 2.300 | 3.100 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 367588 | |
| <I/σ(I)> | 41.7 | 8.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 31.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 35 % ETHYLENE GLYCOL |
