1W45
The 2.5 Angstroem structure of the K16A mutant of annexin A8, which has an intact N-terminus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-31 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.297, 59.653, 70.788 |
| Unit cell angles | 84.42, 83.32, 74.10 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 26449 | |
| <I/σ(I)> | 7.58 | 2.04 |
| Completeness [%] | 0.9 | 0.9 |
| Redundancy | 1.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M TRIS PH 7.5, 2M AMMONIUM SULPHATE, 2% PEG 400 |
