1W3Y
Crystal structure of S. pneumoniae hyaluronate lyase in complex with palmitoyl-vitamin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.131, 102.714, 102.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.910 - 1.650 |
| R-factor | 0.1924 |
| Rwork | 0.192 |
| R-free | 0.21000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.880 |
| Number of reflections | 106633 | |
| <I/σ(I)> | 22.8 | 1.3 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 62% SATURATED AMMONIUM SULFATE, 100 MM SODIUM CACODYLATE PH6.00, 100 MM SODIUM CHLORIDE, 10 MM EDTA, 2% DIOXANE |
