1W2W
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.620, 105.450, 263.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.218 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.870 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 169966 | |
| <I/σ(I)> | 11.6 | 4.1 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.2 M AMMONIUM SULPHATE, 25 % PEG3350, 0.1 M BISTRIS, PH 5.5, 20 DEG C |
