1W1H
Crystal Structure of the PDK1 Pleckstrin Homology (PH) domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-03 |
| Detector | RIGAKU RAXIS4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.690, 65.276, 101.123 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDK1PH INSP4 MODEL |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.576 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 95063 | |
| <I/σ(I)> | 13.2 | 2.7 |
| Completeness [%] | 97.5 | 80.1 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30 % PEG 5000 MME, 0.2 M AMMONIUM SULPHATE, 0.1 M MES [PH 6.5] |
