1VZO

The structure of the N-terminal kinase domain of MSK1 reveals a novel autoinhibitory conformation for a dual kinase protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Collection date	2001-06-15
Spacegroup name	P 21 21 21
Unit cell lengths	66.629, 73.772, 89.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.208
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1atp
RMSD bond length	0.013
RMSD bond angle	1.409
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.307
Number of reflections	34183
<I/σ(I)>	23.6	4.5
Completeness [%]	94.9	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5		2.0M AMMONIUM SULPHATE, 0.1M TRIS PH8.5, pH 9.50