1VZD

L. CASEI THYMIDYLATE SYNTHASE MUTANT E60Q TERNARY COMPLEX WITH FDUMP AND CB3717

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-04-05
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 61 2 2
Unit cell lengths	78.500, 78.500, 230.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.188
Rwork	0.188
RMSD bond length	0.022
RMSD bond angle	27.580
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.115
Number of reflections	10269
Completeness [%]	60.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	potassium phosphate	20 (mM)
2	1	drop	enzyme	10 (mg/ml)
3	1	drop	ammonium sulfate	15-20 (mM)
4	1	drop	dUMP	5 (mM)
5	1	drop	potassium phosphate	20 (mM)