1VZ7
Ornithine Acetyltransferase (ORF6 Gene Product - Clavulanic Acid Biosynthesis) from Streptomyces clavuligerus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.989, 180.344, 73.030 |
Unit cell angles | 90.00, 89.88, 90.00 |
Refinement procedure
Resolution | 67.690 - 3.000 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.31300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.420 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.104 | 0.269 |
Number of reflections | 29519 | |
<I/σ(I)> | 4 | 2.9 |
Completeness [%] | 93.6 | 99.2 |
Redundancy | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | PROTEIN AT 12MG/ML MIXED 1:1 WITH A SOLUTION OF: 1.2M (NH4)2SO4, 40MM NH4H2PO4, 0.1M TRIS PH 8.0, 6% GLYCEROL. |