1VYW
Structure of CDK2/Cyclin A with PNU-292137
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-20 |
Detector | ADSC CCD |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 184.223, 184.223, 214.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.860 - 2.300 |
Rwork | 0.229 |
R-free | 0.25800 |
Structure solution method | OTHER |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNX |
Refinement software | CNX (2002) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.690 |
Number of reflections | 94577 | |
<I/σ(I)> | 16 | 2.3 |
Completeness [%] | 99.0 | 97 |
Redundancy | 4.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% AMMONIUM SULPHATE, 1M KCL, 40MM HEPES PH 7 |