1VYB

Endonuclease domain of human LINE1 ORF2p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	91.044, 126.477, 43.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.100 - 1.800
R-factor	0.188
Rwork	0.186
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dew
RMSD bond length	0.017
RMSD bond angle	1.661
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.062	0.442
Number of reflections	685558
<I/σ(I)>	17.2	2.4
Completeness [%]	97.5	90.2
Redundancy	15.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.00