1VYA
Identification and characterization of the first plant G-quadruplex binding protein encoded by the Zea mays L. nucleoside diphosphate1 gene, ZmNDPK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2013-11-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.962, 179.198, 88.469 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 39.859 - 2.050 |
R-factor | 0.1609 |
Rwork | 0.160 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u8w |
RMSD bond length | 0.007 |
RMSD bond angle | 1.013 |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.120 | 0.660 |
Number of reflections | 123697 | |
<I/σ(I)> | 19.8 | 1.75 |
Completeness [%] | 96.3 | 92.3 |
Redundancy | 7.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate (pH 6.5), 12% PEG 8000, and 200 mM CaCl2 |