1VY4

Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing acylated tRNA-substrates in the A and P sites.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	209.450, 448.850, 619.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.896 - 2.600
R-factor	0.2245
Rwork	0.223
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.982
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.425	224.425	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.232	0.022	0.022
Number of reflections	1760252	20353	129619
<I/σ(I)>	8.58	29.5	0.97
Completeness [%]	99.9	97.8	99.8
Redundancy	9.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K