1VVJ
Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.546, 449.211, 619.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.811 - 3.440 |
| R-factor | 0.245027068825 |
| Rwork | 0.243 |
| R-free | 0.28452 |
| Starting model (for MR) | 3I9D AND 3I93 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.820 | 34.820 | 3.340 |
| High resolution limit [Å] | 3.260 | 14.580 | 3.260 |
| Rmerge | 0.195 | 0.012 | 0.012 |
| Number of reflections | 881904 | 9461 | 62649 |
| <I/σ(I)> | 6.57 | 30.36 | 1.05 |
| Completeness [%] | 98.1 | 87.9 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K |
