1VSU
Crystal Structure of Apo-glyceraldehyde 3-phosphate dehydrogenase from Cryptosporidium parvum
Replaces: 3CHZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | MAR300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.359, 121.646, 80.003 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 39.970 - 2.200 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k3t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 68051 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.8 | 97.5 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 20% PEG 3000, pH 7.5, vapor diffusion, hanging drop, temperature 298K, pH 7.50 |
