1VSU
Crystal Structure of Apo-glyceraldehyde 3-phosphate dehydrogenase from Cryptosporidium parvum
Replaces: 3CHZExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-07-02 |
Detector | MAR300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.359, 121.646, 80.003 |
Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
Resolution | 39.970 - 2.200 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k3t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 68051 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 98.8 | 97.5 |
Redundancy | 2.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 20% PEG 3000, pH 7.5, vapor diffusion, hanging drop, temperature 298K, pH 7.50 |