1VR3
Crystal structure of Acireductone dioxygenase (13543033) from Mus musculus at 2.06 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-10 |
Detector | ADSC |
Wavelength(s) | 0.979735,0.979562,0.956885 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 79.136, 79.136, 114.128 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.530 - 2.060 |
R-factor | 0.16387 |
Rwork | 0.162 |
R-free | 0.19532 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.436 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.540 | 2.170 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 23148 | |
<I/σ(I)> | 13.6 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5.6 | 277 | 5.0% Glycerol, 19.0% iso-Propanol, 19.0% PEG-4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5.6 | 277 | 5.0% Glycerol, 19.0% iso-Propanol, 19.0% PEG-4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |