1VPX
Crystal structure of Transaldolase (EC 2.2.1.2) (TM0295) from Thermotoga maritima at 2.40 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-17 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.298, 104.419, 171.124 |
| Unit cell angles | 90.00, 108.99, 90.00 |
Refinement procedure
| Resolution | 83.130 - 2.400 |
| R-factor | 0.19884 |
| Rwork | 0.196 |
| R-free | 0.24694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6w |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.508 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4 4.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.190 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 162044 | |
| <I/σ(I)> | 9.1 | 1.3 |
| Completeness [%] | 85.9 | 56.1 |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7 | 293 | 30.0% 1,2-propanediol, 0.2M (NH4)2SO4, 10.0% Glycerol, 0.1M HEPES, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 7.0 |
