1VPW

STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	175.890, 94.930, 81.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.174 *
Rwork	0.174
RMSD bond length	0.013
RMSD bond angle	20.930 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.900
High resolution limit [Å]	2.700	2.700
Rmerge	0.068 *
Total number of observations	71433 *
Number of reflections	18486
<I/σ(I)>	8.8
Completeness [%]	98.0	79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Schumacher, M.A., (1994) J.Mol.Biol, 242, 302. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
10	1	reservoir	PEG4000	25 (%)
11	1	reservoir	ammonium sulfate	0.4 (M)
12	1	reservoir	cobalt hexammine	50 (mM)
13	1	reservoir	ammonium phosphate	0.1 (M)
2	1	drop	dithiothreitol	1.0 (mM)
3	1	drop	sodium potassium phosphate	160 (mM)
4	1	drop	DNA	0.6 (mM)
5	1	drop	sodium cacodylate	50 (mM)
6	1	drop	PEG4000	25 (%)
7	1	drop	ammonium sulfate	0.4 (M)
8	1	drop	cobalt hexammine	50 (mM)
9	1	drop	ammonium phosphate	0.1 (M)