1VLR
Crystal structure of mRNA decapping enzyme (DcpS) from Mus musculus at 1.83 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-08 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.399, 59.231, 100.140 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 37.580 - 1.830 |
| R-factor | 0.16053 |
| Rwork | 0.158 |
| R-free | 0.20101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1st0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.667 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.580 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 64852 | |
| <I/σ(I)> | 18.07 | 3.04 |
| Completeness [%] | 97.9 | 84.84 |
| Redundancy | 3.69 | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.2 | 277 | 0.2M KF, 20.0% PEG-3350, No Buffer pH 7.2, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
