1VL0
CRYSTAL STRUCTURE OF A DTDP-4-DEHYDRORHAMNOSE REDUCTASE, RFBD ORTHOLOG (CA_C2315) FROM CLOSTRIDIUM ACETOBUTYLICUM ATCC 824 AT 2.05 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-10 |
Detector | ADSC |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.800, 130.670, 151.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.050 |
R-factor | 0.17072 |
Rwork | 0.169 |
R-free | 0.20701 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.529 |
Data reduction software | MOSFLM |
Data scaling software | SCALA ((CCP4 4.2)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.990 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.076 | 0.572 |
Number of reflections | 66292 | |
<I/σ(I)> | 12.5 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 0.06M MES, 0.04M MES_Na, 2% NP_PEG MME 2000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 0.06M MES, 0.04M MES_Na, 2% NP_PEG MME 2000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 0.06M MES, 0.04M MES_Na, 2% NP_PEG MME 2000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |