1VKP

X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT5G08170, AGMATINE IMINOHYDROLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-04
Detector	MAR CCD 165 mm
Spacegroup name	P 1 21 1
Unit cell lengths	58.020, 115.813, 66.634
Unit cell angles	90.00, 97.10, 90.00

Refinement procedure

Resolution	19.880 - 1.530
R-factor	0.148
Rwork	0.146
R-free	0.17400
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.556
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.580
High resolution limit [Å]	1.530	1.530
Rmerge	0.091	0.322
Number of reflections	127993
<I/σ(I)>	11.69	3.23
Completeness [%]	98.2	96.3
Redundancy	7.9	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	297	10 mg/ml protein, 18% PEG 4000, 8% ethylene glycol, 100 mM MOPS, Vapor diffusion, hanging drop, pH 7.00, temperature 297K