1VK2
Crystal structure of Uracil-DNA glycosylase (TM0511) from Thermotoga maritima at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-03 |
| Detector | ADSC |
| Wavelength(s) | 1.0000, 0.9796, 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.049, 61.049, 127.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.090 - 1.900 |
| R-factor | 0.17886 |
| Rwork | 0.177 |
| R-free | 0.20573 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.671 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19816 | |
| <I/σ(I)> | 25.15 | 2.03 |
| Completeness [%] | 100.0 | 99.95 |
| Redundancy | 7.87 | 6.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8.5 | 277 | TRIS pH 8.5, 8% PEG-8000, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K. cryo condition: 25% PEG-200. |
