1VJB

crystal structure of the ligand-binding domain of the estrogen-related receptor gamma in complex with 4-hydroxytamoxifen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-22
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	74.624, 74.624, 144.778
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.130 - 3.200
R-factor	0.256
Rwork	0.256
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kv6
RMSD bond length	0.010
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	8116
<I/σ(I)>	30.9	4.2
Completeness [%]	99.0	100
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8.00