1VJ7
Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.490, 45.450, 126.470 |
| Unit cell angles | 90.00, 109.83, 90.00 |
Refinement procedure
| Resolution | 22.700 - 2.100 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.27200 |
| Structure solution method | MAD + MIR(AS) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.068 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.700 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.395 |
| Number of reflections | 53798 | |
| <I/σ(I)> | 19 | 2.1 |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | PEG 8000, sodium chloride, magnesium chloride, TRIS, MES, GDP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 8.50 |
