1VIO
Crystal structure of pseudouridylate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.163, 76.973, 62.771 |
| Unit cell angles | 90.00, 108.75, 90.00 |
Refinement procedure
| Resolution | 22.100 * - 1.590 |
| Rwork | 0.196 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.450 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 * | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.087 | 0.011 |
| Total number of observations | 587441 * | |
| Number of reflections | 80195 * | |
| <I/σ(I)> | 11.5 | 1.8 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 7.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 277 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-30 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | methionine | 10 (mM) | |
| 5 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 6 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 8 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
