1VIN
BOVINE CYCLIN A3
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.700, 60.900, 83.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.29900 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.410 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.068 |
Number of reflections | 17065 |
Completeness [%] | 91.4 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | cyclin A-3 | 5-10 (mg/ml) | |
2 | 1 | drop | 0.1 (M) | ||
3 | 1 | drop | monothioglycerol | 0.01 (%) | |
4 | 1 | drop | 0.01 (%) | ||
5 | 1 | drop | Tris-HCl | 50 (mM) | |
6 | 1 | drop | reservoir | ||
7 | 1 | reservoir | methoxyPEG5000 | 25-26 (%) | |
8 | 1 | reservoir | 0.2 (M) | ||
9 | 1 | reservoir | Tris-HCl | 0.1 (M) |