1VHR

HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-11-27
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	61.148, 60.040, 52.016
Unit cell angles	90.00, 98.35, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.176
Rwork	0.176
R-free	0.25400
Structure solution method	MIR SOFTWARE USED : PHASES STARTING MODEL FOR MOLECULAR REPLACEMENT: NULL
RMSD bond length	0.016
RMSD bond angle	23.720 *
Data reduction software	MADNES
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	90.000
High resolution limit [Å]	2.100
Rmerge	0.055
Total number of observations	41078 *
Number of reflections	18952
Completeness [%]	86.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	22 *	THE FULL-LENGTH VHR (RESIDUES 2 - 185) WAS CRYSTALLIZED AT A CONCENTRATION OF 5-6 MG/ML WITH 14% POLYETHYLENE GLYCOL 4000, 22.5 MM LITHIUM SULFATE, 50 MM HEPES PH 7.0, AND 0.05% BETA-MERCAPTOETHANOL IN A HANGING DROP OF 20 MICROLITERS. THE DROP WAS EQUILIBRATED AGAINST 1 ML OF TWICE THE CONCENTRATION OF PRECIPITANT SOLUTION AT 23 DEGREE CELSIUS., vapor diffusion - hanging drop, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	VHR	5 (mg/ml)
2	1	drop	PEG4000	10-15 (%)
3	1	drop		22 (mM)
4	1	drop	beta-mercaptoethanol	0.1 (%)
5	1	drop	HEPES	50 (mM)
6	1	reservoir	precipitant		twice the concentration