1VHH
A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Detector technology | IMAGE PLATE |
Collection date | 1995-01-06 |
Detector | FUJI |
Wavelength(s) | 0.9686, 0.9792, 0.9793, 0.9879 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.700, 79.000, 35.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.26100 |
RMSD bond length | 0.009 |
RMSD bond angle | 22.420 * |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.080 |
Number of reflections | 14968 |
Completeness [%] | 86.4 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.4 (mg/ml) | |
2 | 1 | drop | beta-mercaptoethanol | 1.4 (mM) | |
3 | 1 | reservoir | PEG8000 | 30 (%) | |
4 | 1 | reservoir | ammonium sulfate | 0.12 (M) | |
5 | 1 | reservoir | sodium cacodylate | 10 (mM) |