1VH8
Crystal structure of a 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795, 0.9795 , 0.9641 |
Spacegroup name | I 41 |
Unit cell lengths | 104.850, 104.850, 195.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.940 - 2.350 |
Rwork | 0.240 |
R-free | 0.28000 |
Structure solution method | Se-Met MAD phasing |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (4.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.940 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.110 | 0.763 |
Number of reflections | 43488 | |
<I/σ(I)> | 26.3 | 1.6 |
Completeness [%] | 98.8 | 92.5 |
Redundancy | 15.2 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | methionine | 10 (mM) | |
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | drop | protein | 10 (mg/ml) |