1VGA
Structures of unligated and inhibitor complexes of W168F mutant of Triosephosphate Isomerase from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.730, 106.157, 89.526 |
Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
Resolution | 17.200 - 1.800 |
Rwork | 0.207 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ydv |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.210 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.036 | 0.214 |
Completeness [%] | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | PEG1450, Lithium sulfate and Soduim acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |