1VFP
Crystal structure of the SR CA2+-ATPase with bound AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.72 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.920, 123.620, 151.820 |
| Unit cell angles | 90.00, 107.21, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.252 * |
| Rwork | 0.247 |
| R-free | 0.29600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P-domain and M3-M10 transmembrane helices of 1EUL |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.056 | 0.240 |
| Number of reflections | 70956 | |
| <I/σ(I)> | 26.45 | 3.598 |
| Completeness [%] | 99.0 | 94.2 |
| Redundancy | 6.37 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | PEG400, pH 6.1, MICRODIALYSIS |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | glycerol | 20 (%) | |
| 2 | 1 | 1 | PEG400 | 12 (%) | |
| 3 | 1 | 1 | 10 (mM) | ||
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | 2.5 (mM) | ||
| 6 | 1 | 1 | BHT | 0.002 (mg/ml) | |
| 7 | 1 | 1 | dithiothreitol | 0.2 (mM) | |
| 8 | 1 | 1 | MES | 20 (mM) | |
| 9 | 1 | 1 | AMPPCP | 0.5 (mM) |
