1VEV

Crystal structure of peptide deformylase from Leptospira Interrogans (LiPDF) at pH6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-01
Detector	MARRESEARCH
Wavelength(s)	1.20
Spacegroup name	P 41 21 2
Unit cell lengths	82.192, 82.192, 201.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.910 - 2.510
R-factor	0.258
Rwork	0.258
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB id 1SV2
RMSD bond length	0.008
RMSD bond angle	1.384
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.910	2.660
High resolution limit [Å]	2.510	2.500
Number of reflections	21663
Completeness [%]	87.7	75.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	4M formate sodium, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K