1VEL

Mycobacterium smegmatis Dps tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2003-06-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	123.643, 123.643, 262.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.210 - 2.990
R-factor	0.183
Rwork	0.183
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Cubic form of the Mycobacterium smegmatis Dps
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.110
High resolution limit [Å]	2.990	2.990
Rmerge	0.201	0.514
Number of reflections	38007
<I/σ(I)>	7.6
Completeness [%]	90.6	68.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	12.5% PEG 400, 0.02M CdSO4, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K