1VCV
Structure of 2-deoxyribose-5-phosphate aldolase from Pyrobaculum aerophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-06 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 84.540, 84.540, 112.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.070 - 2.000 |
R-factor | 0.2095 |
Rwork | 0.206 |
R-free | 0.24100 |
Structure solution method | MIRAS |
Data reduction software | CrystalClear |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.070 |
High resolution limit [Å] | 2.000 |
Number of reflections | 28389 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% PEG1000, 0.1M ACETATE BUFFER, 0.2M ZINC ACETATE, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |