1VCN

Crystal Structure of T.th. HB8 CTP synthetase complex with Sulfate anion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-27
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	90.410, 117.100, 142.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.250
Rwork	0.248
R-free	0.29200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	35958
Completeness [%]	99.2	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Ammonium Sulfate, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K