1VCC

AMINO TERMINAL 9KDA DOMAIN OF VACCINIA VIRUS DNA TOPOISOMERASE I RESIDUES 1-77, EXPERIMENTAL ELECTRON DENSITY FOR RESIDUES 1-77

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	32.460, 42.510, 60.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.600
R-factor	0.218
Rwork	0.218
R-free	0.28700
RMSD bond length	0.017
RMSD bond angle	1.510
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.590 *
Rmerge	0.069 *	0.205 *
Total number of observations	47905 *
Number of reflections	9781 *	799 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	PEG3300	30 (%)