1VCB

THE VHL-ELONGINC-ELONGINB STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	113
Detector technology	CCD
Collection date	1998-09-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 2 2
Unit cell lengths	93.500, 93.500, 362.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.239 *
Rwork	0.238
R-free	0.28900
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (0.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.070
Total number of observations	123622 *
Number of reflections	41219
Completeness [%]	90.9
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7	4 *	10-15% PEG 2000, 200MM MAGNESIUM ACETATE, 100MM SODIUM CACODYLATE PH 5.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	12-17 (%)	or 10-15% PEG2000
2	1	reservoir	magnesium acetate	0.2 (M)
3	1	reservoir	dithiothreitol	5 (mM)
4	1	reservoir	sodium cacodylate	0.1 (M)