1VCB
THE VHL-ELONGINC-ELONGINB STRUCTURE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1998-09-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 93.500, 93.500, 362.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.239 * |
Rwork | 0.238 |
R-free | 0.28900 |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.070 |
Total number of observations | 123622 * |
Number of reflections | 41219 |
Completeness [%] | 90.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.7 | 4 * | 10-15% PEG 2000, 200MM MAGNESIUM ACETATE, 100MM SODIUM CACODYLATE PH 5.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 12-17 (%) | or 10-15% PEG2000 |
2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
3 | 1 | reservoir | dithiothreitol | 5 (mM) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) |