1V9C
Crystal Analysis of Precorrin-8x Methyl Mutase from Thermus Thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-22 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.571, 67.822, 56.697 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 46.360 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f2v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.042 | 0.322 |
| Number of reflections | 19430 | |
| <I/σ(I)> | 20.9 | 3.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | Lithium sulfate, Sodium citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
