1V84
Crystal structure of human GlcAT-P in complex with N-acetyllactosamine, Udp, and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.974 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.311, 85.782, 122.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.820 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v82 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.443 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.067 | 0.379 |
| Number of reflections | 57093 | |
| <I/σ(I)> | 10.4 | 2.8 |
| Completeness [%] | 97.1 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG5000MME, di-sodium tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
