1V7P
Structure of EMS16-alpha2-I domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.766, 38.449, 69.524 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| Rwork | 0.188 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.209 |
| Number of reflections | 33974 | |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 97.0 | 84.6 |
| Redundancy | 6.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG8000, POTASSIUM DIHYDROGEN PHOSPHATE, SODIUM CHLORIDE, MALONIC ACID, MANGANESE CHLORIDE, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
