1V7N
Human Thrombopoietin Functional Domain Complexed To Neutralizing Antibody TN1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-12-01 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.038, 46.582, 191.359 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 3.300 |
| R-factor | 0.17405 |
| Rwork | 0.167 |
| R-free | 0.29200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iai |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.098 * | 0.214 * |
| Total number of observations | 74809 * | |
| Number of reflections | 35598 | |
| <I/σ(I)> | 3 | 1.1 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 1.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 * | Kuroki, R., (2002) Acta Crystallogr.,Sect.D, 58, 856. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18-20 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG3350 | 19-21 (%(w/v)) | |
| 4 | 1 | reservoir | potassium phosphate | 100 (mM) | pH6.0 |
