1V3U
Crystal structure of leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-21 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.486, 74.591, 79.296 |
| Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
| Resolution | 32.200 - 2.000 |
| Rwork | 0.214 |
| R-free | 0.25700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.102 |
| Number of reflections | 45444 | |
| <I/σ(I)> | 31.7 | 14.5 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 6.5 | 291 | PEG 4000, MES, magnesium chloride, pH 6.5, batch, temperature 291K |
