1V0K
Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-deoxynojirimycin at pH 5.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.080, 46.204, 86.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.150 - 1.030 |
| R-factor | 0.115 |
| Rwork | 0.114 |
| R-free | 0.13700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1od8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.572 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.050 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.047 | 0.415 |
| Number of reflections | 128937 | |
| <I/σ(I)> | 23.8 | 2.31 |
| Completeness [%] | 97.1 | 93.7 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 20 MG/ML PROTEIN, 0.1 M MES, PH 5.8, 18% PEG 5K MME, 7.5% ISOPROPANOL |
