1V0F

Endosialidase of Bacteriophage K1F in complex with oligomeric alpha-2,8-sialic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 2 2 21
Unit cell lengths	99.540, 131.400, 346.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.550
R-factor	0.18263
Rwork	0.180
R-free	0.23150
Structure solution method	OTHER
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.550	2.550
Rmerge	0.107	0.303
Number of reflections	130540
<I/σ(I)>	5.7	2.4
Completeness [%]	88.4	79.8
Redundancy	4.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.50