1V0F
Endosialidase of Bacteriophage K1F in complex with oligomeric alpha-2,8-sialic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 99.540, 131.400, 346.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.550 |
R-factor | 0.18263 |
Rwork | 0.180 |
R-free | 0.23150 |
Structure solution method | OTHER |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.107 | 0.303 |
Number of reflections | 130540 | |
<I/σ(I)> | 5.7 | 2.4 |
Completeness [%] | 88.4 | 79.8 |
Redundancy | 4.3 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |