1V0D

Crystal Structure of Caspase-activated DNase (CAD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Spacegroup name	P 64
Unit cell lengths	110.189, 110.189, 73.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.218
Rwork	0.218
R-free	0.24600
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.389
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Number of reflections	14995
Completeness [%]	94.8
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.00