1UZU

Glycogen Phosphorylase b in complex with indirubin-5'-sulphonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-20
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	128.652, 128.652, 116.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.340 - 2.300
R-factor	0.182
Rwork	0.182
R-free	0.21400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1gfz
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.096	0.439
Number of reflections	42000
<I/σ(I)>	10.5	2.1
Completeness [%]	95.5	75.9
Redundancy	6.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	BES, EDTA, PH 6.7, SMALL TUBES, TEMPERATURE 289K